Cat. No.
MABL-2857
Application
in vitro, in vivo, RBA, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Staphylococcus aureus
Clone No.
4497
From
Recombinant Antibody
Specificity
This antibody is specific for S. aureus β-GlcNAc wall-teichoic acids (WTAs), a pathogen-specific polyanionic glycopolymer that is connected to the thick peptidoglycan layers of Gram-positive bacteria.
Alternative Names
TA; Teichoic acids; WTA; Wall teichoic acids; GlcNAc; N- Acetylglucosamine; β-WTA; β-GlcNAc; β-1,4-GlcNAc; β-O-linked N-acetylglucosamine; β-O-linked GlcNAc sugar modifications on WTA
UniProt
Immunogen
The original antibody was generated by cloning human IgG antibodies from peripheral B cells of patients who had been infected with S. aureus.
Application Notes
The original format of this antibody (human IgG1) was screened for antigen binding specificity using ELISA, and it was successfully used antibody was used in flow cytometry (FC) to label S. aureus (Lehar et al., 2015; PMID: 26536114). This antibody was used in a radioligand binding assay (RBA); affinity analysis and epitope counting were performed by saturation binding analysis using a radio-iodinated version of the antibody (Fong et al., 2018; PMID: 30102105). This antibody was observed to bind strongly to planktonic Wood46 (ATCC 10832) S. aureus cells but very weakly to planktonic LAC∆spa∆sbi (AH4116) S. aureus.
Antibody First Published
Lehar et al. Novel antibody-antibiotic conjugate eliminates intracellular S. Aureus Nature. 2015 Nov 19;527(7578):323-8. doi: 10.1038/nature16057 PMID:26536114
Note on publication
The original publication describes the generation, characterization, and utilization of anti-S. Aureus β-WTA antibodies to treat intracellular reservoirs of Staphylococcus aureus in mice.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
