Cat. No.
MABL-2872
Application
neutralize, ELISA
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
AbD09097
From
Recombinant Antibody
Specificity
This antibody binds mouse sclerostin and cross reacts with human sclerostin and recognizes an epitope adjacent to sclerostin's binding site for the Wnt co-receptor LRP6. Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain which is produced in osteocytes (type of bone cell). The glycoprotein sclerostin has been identified as a negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. Neutralizing anti-sclerostin antibodies are able to restore Wnt activity and enhance bone growth thereby presenting a new osteoanabolic therapy approach for diseases such as osteoporosis.
Alternative Names
SOST
UniProt
Q9BQB4; Q99P68
Immunogen
The original antibody was isolated from the HuCAL GOLD library by panning against mouse sclerostin protein biotinylated using EZ-link Sulfo-NHS-LC-Biotin and coupled to streptavidin-coated magnetic beads.
Application Notes
The binding reactivity of this antibody for mouse sclerostin was tested using ELISA. This antibody binds mouse and human sclerostin with a binding affinity of Kd= 46nM and Kd=260 nM respectively. This antibody efficiently neutralizes sclerostin's Wnt inhibitory activity. In vitro interaction analysis using sclerostin variants revealed that this neutralizing Fab binds to sclerostin's flexible second loop, which has been shown to harbor the LRP5/6 binding motif (PMID: 27558933).
Antibody First Published
Boschert et al. The sclerostin-neutralizing antibody AbD09097 recognizes an epitope adjacent to sclerostin's binding site for the Wnt co-receptor LRP6. Open Biol. 2016 Aug;6(8):160120. PMID:27558933
Note on publication
This paper describes the generation of antibodies against human and mouse sclerostin.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
