Cat. No.
MABL-2898
Application
Neutralisation, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Escherichia coli
Clone No.
5-5B
From
Recombinant Antibody
Specificity
This antibody is specific for the Stx1 B subunit, and does not recognise Stx2
Alternative Names
Stx1; stx1B
UniProt
Q8X4M7
Immunogen
This antibody was raised by immunising mice with Stx1 toxoid.
Application Notes
This antibody has been shown to bind the B subunit of Stx1 in Western blot analysis (Nakao et al, 2002). This antibody is able to neutralise Stx1 cytotoxicity (Nakao, 2002). Flow cytometric experiments have revealed that this antibody binds to free Stx1 B subunits, not cell-bound Stx1, and inhibits Stx1 binding to its receptor on target cells (Nakao et al, 2002). A Fab derived from this antibody also specifically binds Stx1, as determined by ELISA, and neutralises Stx1 cytotoxicity (Inoue et al, 2004).
Antibody First Published
Nakao et al. Monoclonal Antibody to Shiga Toxin 1, Which Blocks Receptor Binding and Neutralizes Cytotoxicity Microbiol. Immunol., 46(11), 777―780, 2002 PMID:12516775
Note on publication
Describes the original generation of this antibody, and its use in neutralisation assays and Western blot and FC experiments.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
