Cat. No.
MABL-2899
Application
IP, neutralize, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Bacteriophage H30
Clone No.
13C4
From
Recombinant Antibody
Specificity
This antibody is specific for the B subunit of Shiga-like toxin 1. This antibody does not bind B polypeptide of Stx2. Stx 1 is a toxin produced by Shiga toxin (Stx)-producing E. coli (STEC) strains. Infections with STEC can lead to diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS).
Alternative Names
SLT1; stxB; SLT-1 B subunit; SLT-1b; SLT-Ib; Shiga-like toxin 1 subunit B; Verocytotoxin 1 subunit B; Verotoxin 1 subunit B
UniProt
P69178
Immunogen
The original antibody was generated by immunization of BALB/c mice (female, 6 to 8 weeks old) with purified, biologically active Shiga-like toxin (SLT) from E. coli H30.
Application Notes
The original IgG1 antibody neutralized the cytotoxicity of E. coli Shiga-like toxin (SLT) and were able to immunoprecipitate intact labeled toxin with Staphylococcus aureus protein A. The original IgG1 antibody neutralizes Shiga toxin from Shigella dysenteriae type 1 and Shiga-like toxic activities in crude cell extracts from Shigella flexneri, Vibrio cholerae, Vibrio parahaemolyticus, and Salmonella typhimurium. The IgG1 version of this antibody was also used in a colony blot assay for the detection of bacterial colonies which produce high levels of SLT (PMID: 3905611). This antibody neutralizes the cytotoxic and lethal activities of Stx1, This antibody was also reported to block the binding of Stx1 to its receptor, globotriaosyl ceramide by binding three nonlinear regions of the molecule that span receptor recognition sites on StxB1, one of which includes the essential residue 55N. This antibody was able to identify histidine- tagged StxB1 in a western blot assay. It did not recognize Stx1d (PMID: 17030576). This antibody was used in a sandwich ELISA as a detector antibody to identify production of Stx1 by E. coli strain MHI 813 (PMID: 12734256).
Antibody First Published
Strockbine et al. Characterization of monoclonal antibodies against Shiga- like toxin from Escherichia coli. Infect Immun. 1985 Dec;50(3):695-700. PMID:3905611
Note on publication
Describes the generation and characterization of three neutralizing antibodies against Shiga-like toxin (SLT) from Escherichia coli.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
