Cat. No.
MABL-2900
Application
immunotherapy, IP, neutralize, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Escherichia phage 933W (Bacteriophage 933W)
Clone No.
11E 10
From
Recombinant Antibody
Specificity
This antibody binds a conformational epitope on the A subunit of Shiga-like toxin 2 comprising region A (StxA2 residues 42 to 49), region B (StxA2 residues 96 to 100), and region C (StxA2 residues 244 to 259) (PMID: 19433543). This antibody does not bind A subunit of Stx1. StxII is a toxin produced by Shiga toxin (Stx)-producing E. coli (STEC) strains. Infections with STEC can lead to diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS).
Alternative Names
SLT2; stxA2; stx2A; SLT-2 A subunit; SLT-2a; SLT-IIa; Shiga-like toxin 2 subunit A; Verocytotoxin 2 subunit A; Verotoxin 2 subunit A; rRNA N-glycosidase 2
UniProt
P09385
Immunogen
The original antibody was generated by immunizing 4 to 8 week old female BALB/c mice with 25µg of formalinized StxII toxoid or 25µg of glutaraldehyde treated toxoid in complete Freund adjuvant.
Application Notes
The binding characterization of the original IgG1 version of this antibody towards Shiga like toxin 2 was done using ELISA. The original IgG1 version of this antibody was also used to immunoprecipitate SLT-Il toxoid. This antibody is capable of neutralizing the cytotoxicity of SLT-ll but not of SLT-I, Shiga toxin, or a variant of SLT-II produced by E. coli that causes edema disease of swine (PMID: 3053764). This antibody was also used a primary antibody in the detection of SLT-II in a western blot assay. It was reported that binding of 11E10 to Stx2 prevented the toxin from inhibiting protein synthesis in an in vitro assay but also altered the overall cellular distribution of Stx2 in Vero cells. It was suggested that binding of antibody 11E10 to Stx2 neutralizes the effects of the toxin by preventing the toxin from reaching and/or inactivating the ribosomes (PMID: 19433543).
Antibody First Published
Perera et al. Isolation and characterization of monoclonal antibodies to Shiga-like toxin II of enterohemorrhagic Escherichia coli and use of the monoclonal antibodies in a colony enzyme-linked immunosorbent assay. J Clin Microbiol. 1988 Oct;26(10):2127-31. PMID:3053764
Note on publication
Describes the generation of 5 neutralizing antibodies for Shiga-like toxin 2.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
