Cat. No.
MABL-2902
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Escherichia phage 933W (Bacteriophage 933W)
Clone No.
S2D8
From
Recombinant Antibody
Specificity
This antibody binds the A subunit of Shiga-like toxin 2. Stx2 is a toxin produced by Shiga toxin (Stx)-producing E. coli (STEC) strains. Infections with STEC can lead to diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS).
Alternative Names
SLT2; stxA2; stx2A; SLT-2 A subunit; SLT-2a; SLT-IIa; Shiga-like toxin 2 subunit A; Verocytotoxin 2 subunit A; Verotoxin 2 subunit A; rRNA N-glycosidase 2
UniProt
P09385
Immunogen
The original antibody was generated by immunizing BALB/c mice with StxII protein mixed with an equal volume of Freund’s complete adjuvant.
Application Notes
This antibody can be used as a capture antibody in the detection of shiga like toxin 2 subunit A in a sample using double antibody sandwich ELISA. The detection can be done using S2C6 antibody (Shi et al, 2016; CN103235140).
Antibody First Published
Shi et al. Establishment of a double-antibody sandwich ELISA for the detection of shiga toxin typeⅡin shiga toxin-producing Escherichia coli infection. Chinese Journal of Microbiology and Immunology 2016;36(10):771-774
Note on publication
Describes a double-antibody sandwich ELISA method for detection of shiga toxin typeⅡ ( StxⅡ) using two antibodies S2C6 and S2D8.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
