Cat. No.
MABL-2906
Application
IP, WB, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Pig (Sus scrofa)
Clone No.
3B11 (3B11/11)
From
Recombinant Antibody
Specificity
This antibody recognizes and binds porcine sialoadhesin.
Alternative Names
CD169; pSn; Sn; p210; Siglec-1; Sialic acid-binding Ig-like lectin 1
UniProt
A7LCJ3
Immunogen
The original antibody was generated by immunizing BALB/c mice with porcine alveolar macrophages.
Application Notes
This antibody is recommended to be used as a tool in the study of porcine macrophage maturation and differentiation and for determining their heterogeneity both in normal and pathological conditions. This antibody can recognize porcine sialoadhesin in a western blot assay and an immunoprecipitation assay (PMID: 9151393). Tissue distribution of the antigen recognized by this mAb was analyzed by immunohistochemical staining of different tissues (PMID:19081005). 3B11 successfully binds alveolar macrophages and PAM cells in a flow cytometric analysis (PMID:19081005, 23785195). Porcine sialoadhesin has also been analyzed as potential antigen targeting receptors using mouse IgG as surrogate antigens (PMID:21557980). The mAb was also used in the study the role of sialoadhesin in porcine reproductive and respiratory syndrome virus infection using immunoflurosence and flow cytometric analysis (Liu et al, 2020)
Antibody First Published
Bullido et al. Monoclonal antibodies specific for porcine monocytes/macrophages: macrophage heterogeneity in the pig evidenced by the expression of surface antigens. Tissue Antigens (1997); 49(4):403-13. PMID:9151393
Note on publication
Describes the distribution of surface antigens on porcine monocytes and macrophages using monoclonal antibodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
