Cat. No.
MABL-2911
Application
crystallization, IP, RIA, FC
Isotype
Engineer antibody
Species Reactivity
Reovirus strain T3D
Clone No.
9BG5
From
Recombinant Antibody
Specificity
9BG5 was identified as a clone which significantly neutralised the strain T3D reovirus Sigma1 as assayed using viral titre of infection (Burstin et al, 1982). 9BG5 was able to immunoprecipitate strain T3D Sigma1, however some cross-reactivity was observed with strain T1L reoviral Sigma1 (Burstin et al, 1982; Dietrich et al, 2017). Recently, the interaction between 9BG5 and strain T3D reoviral Sigma1 was shown crystallographically (Dietrich et al, 2017). 9BG5 binds to the Sigma1 head region, with 5 CDRs contacting a relatively flat, contiguous epitope.
Alternative Names
T3D HA; Outer capsid protein sigma1; sigma1 protein; Reovirus strain T3D sigma 1; strain T3D S1; T3D S1; type 3 haemagglutinin; type 3 HA
UniProt
P03528
Immunogen
Murine antibodies were produced by immunising BALB/c mice with an injection of strain TD3 reovirus. Splenocytes from immunised mice were obtained and fused with the NS1 mouse myeloma cell line to generate a hybridoma.
Application Notes
9BG5 effectively neutralised reovirus strain T3D activity, and showed some inhibition of erythrocyte haemagglutination in virus-erythrocyte suspensions (Burstin et al, 1982). 9BG5 was also used to immunoprecipitate radiolabelled viral Sigma1 from L929 mouse fibroblast supernatants. More recently, 9BG5 was shown to cross-react with Sigma1 from the reovirus strain T1L, and contributed to blocking adherence of both T1L and T3D virions to CHO cells shown by flow cytometry (Dietrich et al, 2017).
Antibody First Published
Burstin et al. Evidence for functional domains on the reovirus type 3 hemagglutinin. Virology. 1982 Feb;117(1):146-55. PMID:6175082
Note on publication
Describes the generation of a panel of anti-reovirus (strain T3D) mAbs using generated murine hybridomas which recognised different functional epitopes of the viral Sigma1 protein.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
