Cat. No.
MABL-2912
Application
SPRIA, IF
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
Jel 103
From
Recombinant Antibody
Specificity
Jel 103 is highly specific for single-stranded RNA and shows sequence specificity as it binds to poly(rI) but not to poly(dI), poly(rG) or to any other homopolymer - it does not bind randomly to calf thymus DNA. Jel 103 Fab is shown to form base stacking interactions with a Tyr residue and two salt-bridge interactions to the phosphate backbone with Lys and Arg residues.
Alternative Names
polyriboinosinic acid; single stranded RNA; single-stranded RNA; inosine
UniProt
Immunogen
Jel 103 was selected from a hybridoma cell line generated by the fusion of the MOPC 315-43 myeloma cell line with mouse spleen cells derived from C57/B1 mice which had been immunized with poly(rI)poly(dC) duplex complexed to methylated bovine serum albumin.
Application Notes
Jel 301 can be used for the detection of single-stranded poly(rI) and for binding studies where the antibody has been used previosly in solid-phase radioimmunoassays.
Antibody First Published
Pokkuluri et al. Preparation, characterization and crystallization of an antibody Fab fragment that recognizes RNA. Crystal structures of native Fab and three Fab-mononucleotide complexes. J Mol Biol. 1994 Oct 21;243(2):283-97. PMID:7523684
Note on publication
Describes the generation of jel 301 fab fragment and the structures of the fab in complex with rIP, rGDP and dIMP are reported.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
