Cat. No.
MABL-2913
Application
Block, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human, Cynomolgus Monkey
Clone No.
12G6
From
Recombinant Antibody
Specificity
This antibody binds human and cynomolgus monkey SIRP alpha, which is a regulatory membrane glycoprotein and an immunoglobulin-like cell surface receptor for CD47. This protein plays a key role in intracellular signaling during synaptogenesis and in synaptic function. Cancer cells highly express CD47 that activates SIRPα and inhibit macrophage-mediated destruction.
Alternative Names
CD172a; SIRPα; SIRPA; Signal regulatory protein α; Signal regulatory protein alpha; Tyrosine-protein phosphatase non-receptor type substrate 1; SHP substrate 1; SHPS-1; Brain Ig-like molecule with tyrosine-based activation motifs; Bit; CD172 antigen-like family member A; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MyD-1 antigen; Signal-regulatory protein alpha-1; Sirp-alpha- 1; Signal-regulatory protein alpha-2; Sirp-alpha-2; Signal-regulatory protein alpha-3; Sirp-alpha-3; p84
UniProt
P78324
Immunogen
The original antibody was generated by immunizing BALB/c mice with recombinant human SIRPα-hFc fusion protein.
Application Notes
The binding reactivity of this antibody towards recombinant human SIRPα-his fusion protein was checked using ELISA. Flow cytometry was used to detect the binding of the antibody to SIRPα on the membrane of human and cynomolgus monkey SIRPα overexpressed cells. This antibody binds cynomolgus monkey SIRPα with a weaker binding affinity than human SIRPα. This antibody can effectively block the binding of CD47 protein to cell surface SIRPα (CN110734897).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
