Cat. No.
MABL-2914
Application
binding assay, IP, WB, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
SAF10.1
From
Recombinant Antibody
Specificity
This antibody recognises an epitope in the membrane most distal domain (D1) of SIRP alpha. It crossreacts with other SIRPs.
Alternative Names
Tyrosine-protein phosphatase non-receptor type substrate 1; SHP substrate 1; SHPS-1; Brain Ig-like molecule with tyrosine-based activation motifs; Bit; CD172 antigen-like family member A; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MyD-1 antigen; Signal-regulatory protein alpha-1; Sirp-alpha-1; Signal-regulatory protein alpha-2; Sirp-alpha-2; Signal-regulatory protein alpha-3; Sirp-alpha-3; p84; CD172a
UniProt
P78324
Immunogen
This antibody was raised by immunisisng BALB/c mice with an eukaryotically expressed SIRPα-Fc fusion protein consisting of the extracellular domain and emulsified with complete Freund’s adjuvant and followed by two subsequent boosts of SIRPα-Fc fusion protein emulsified with IFA (Lee et al., 2007).
Application Notes
SAF10.1, can be used to detect SIRPs in a wide variety of tissues and cell types. For instance, this antibody was successfully used to detect SIRPs from COS7.2 cell lysates via Western blot (Lee et al., 2008).
Antibody First Published
Lee et al. Novel structural determinants on SIRP alpha that mediate binding to CD47. J Immunol. 2007 Dec 1;179(11):7741-50. PMID:18025220
Note on publication
This article describes the generation and characterisation of the SAF10.1 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
