Cat. No.
MABL-2915
Application
IP, Block, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
OX-119
From
Recombinant Antibody
Specificity
Hybridoma supernatants were initially screened by ELISA, using SIRPg-CD4, human SIRPa-Fc, human SIRPb-Fc or CD4 to eliminate hybridomas with cross-reactivity to rat CD4, human SIRPa, and SIRPb. There is some marginal binding of OX-119 to SIRPb transfected 293T cells. SIRPg binds CD47. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation. Expressed on CD4+ T- cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells..
Alternative Names
CD172g; OX119; MRC OX-119; MRC OX119; SIRP-g; SIRPg; SIRP gamma; SIRP-gamma; CD172 antigen-like family member B; signal-regulatory protein beta; CD172-g
UniProt
Q9P1W8
Immunogen
OX-119 was prepared by immunizing BALB/c mice with a chimeric protein made up of human SIRPg and a leader peptide of rat CD4 domain (SIRPg-CD4 ).
Application Notes
OX-119 is able to perform staining of peripheral blood myeloid cells (PBMCs) and block SIRPg-mediated apoptosis. OX-119 can also be used in IP, FC and ELISA.
Antibody First Published
Brooke et al Human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) family. J Immunol 173:2562-70 2004 PMID:15294972
Note on publication
Describes the determintion of OX-119 specificity by ELISA, FC and IP and decribes use of OX-119 to observe the distribution of SIRPg expression and its ability to block SIRPg-mediated apoptosis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
