Cat. No.
MABL-2941
Application
SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
1H3-1
From
Recombinant Antibody
Specificity
This antibody binds SN-38, which is an active metabolite of irinotecan (a semisynthetic analog of camptothecin - a topoisomerase I inhibitor) generated by carboxyl esterases through hydrolysis. SN-38 has potential antineoplastic activity. It binds and inhibits topoisomerase I by stabilizing the cleavable complex between topoisomerase I and DNA, resulting in DNA breaks, inhibition of DNA replication, and apoptosis. In vitro cytotoxicity assays have shown that SN-38 is 1,000-times more cytotoxic than irinotecan against various cancerous cells. SN-38 is commonly used as a cytotoxic drug payload in the ADC field.
Alternative Names
SN38; Topotecan; Camptothecin; CPT; 9-[(dimethylamino)methyl]-10- hydroxy-(4S)-camptothecin; 10-Hydroxy-7-ethylcamptothecin; 7-Ethyl-10-hydroxy-20(S)-camptothecin; 7- Ethyl-10-hydroxycamptothecin; NK 012; NK-012
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with SN38-BSA conjugate.
Application Notes
The binding reactivity of this antibody to SN38-BSA conjugate was determined using ELISA. The binding affinity of this antibody to SN38-KLH conjugate was determined using surface plasmon resonance (SPR). It was reported that this antibody binds SN38-KLH conjugate with a binding affinity of Kd=3.06 nM (CN115197323).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
