Cat. No.
MABL-2942
Application
neutralize, ELISA
Isotype
Engineer antibody
Species Reactivity
Bothrops asper (Terciopelo), Bothrops neuwiedi
Clone No.
MABaP1
From
Recombinant Antibody
Specificity
This antibody reacts with snake venom metalloproteinase (SVMP) BaP1 present in the venom of Bothrops asper. BaP1 is a P-I class of SVMP relevant in the local tissue damage associated with envenomations by Bothrops asper. This metalloproteinase exhibits a weak hemorrhagic activity and rapidly degrades the Aalpha-chain (FGA) of fibrinogen, and later on, degrades the Bbeta-chain (FGB) of fibrinogen.
Alternative Names
BaP1; Hemorrhagic metalloproteinase BaP1; BnP1
UniProt
P83512
Immunogen
The original antibody was generated by immunizng BALB/c mice with 10 mg BaP1 obtained from B. asper venom emulsified in incomplete Marcol/Montanide adjuvant.
Application Notes
The antibody can bind snake venom metalloproteinases BaP1, whole venom of B. asper and BnP1 (B. neuwiedi) in an ELISA. This antibody completely neutralized in vivo hemorrhage, muscle necrosis, and inflammation induced by the toxin. This antibody completely neutralize myotoxicity induced by BaP1. In general it was capable of neutralizing biological effects of this SVMP (PMID: 20674587; 20674587).
Antibody First Published
Fernandes et al. Immunochemical and biological characterization of monoclonal antibodies against BaP1, a metalloproteinase from Bothrops asper snake venom. Toxicon. 2010 Nov;56(6):1059-65. doi: 10.1016/j.toxicon.2010.07.014. PMID:20674587
Note on publication
Describes the generation of a neutralizing antibody against snake venom metalloproteinase BaP1 of Bothrops asper.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
