Cat. No.
MABL-2946
Application
in vitro, WB
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
B12
From
Recombinant Antibody
Specificity
This antibody is specific for misfolded SOD1.
Alternative Names
SOD1; SOD1G93A; SOD1 A4V
UniProt
Immunogen
This antibody was raised from a phage-displayed scFv antibody library
Application Notes
This antibody was used for a western blot on reacted against denatured wt and mt SOD1. It reacted to denatured wt, A4V and G93A SOD1 proteins but not the control SH3 protein. This antibody was used in vitro on NSC cells to determine the effect of the antibody on A4V and G93A mtSOD1 mediated cell death. The antibody has shown the ability to decrease the cell death caused by A4V and G93A mtSOD1 (Ghadge et al., 2014; PMID:23607939).
Antibody First Published
Ghadge et al. Single chain variable fragment antibodies block aggregation and toxicity induced by familial ALS-linked mutant forms of SOD1 Neurobiol Dis. 2013 Aug; 56: 74–78. PMID:23607939
Note on publication
The original paper generated single chain fragments of variable region antibodies (scFvs) against SOD1, and then expressed them as ‘intrabodies’ within a motor neuron cell line.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
