Cat. No.
MABL-2948
Application
in vivo, IP, WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
D3H5
From
Recombinant Antibody
Specificity
This antibody was specific for misfolded SOD1 and does not bind to WT SOD1.
Alternative Names
SOD1; SOD1G93A; SOD1 A4V
UniProt
Immunogen
This antibody was raised by immunizing mice with a recombinant apo form of G93A human SOD1
Application Notes
The original antibody was used for an ELISA on SOD1 WT and misfolded SOD1. It only showed binding to the misfolded SOD1 protein. This antibody was used for a western blot on SOD1 WT and misfolded SOD1. This antibody was expressed in the spinal cords of mice with a SOD1 mutation simulating ALS. It showed that it delayed disease onset and extended lifespans (Patel et al., 2014; PMID:24394188). This antibody was used for immunohistochemistry on spinal cords from transgenic mice over-expressing the G93A-SOD1 mutation (Gros-Louis et al., 2010; PMID:20345765), and on SOD1G93A rat spinal cords (Pickles et al., 2016; PMID:27121871). The antibody was used for immunoprecipitation on spinal cords from transgenic mice over-expressing the G93A-SOD1 mutation. This antibody was used in vivo on G93A-SO D1 mice. It delayed the mortality on these mice (Gros-Louis et al., 2010; PMID:20345765).
Antibody First Published
Gros-Louis et al. Intracerebroventricular infusion of monoclonal antibody or its derived Fab fragment against misfolded forms of SOD1 mutant delays mortality in a mouse model of ALS J Neurochem. 2010 Jun;113(5):1188-99. PMID:20345765
Note on publication
In the original paper a passive immunization approach based on intracerebroventricular infusion in G93A-SOD1 mice of monoclonal antibodies specific to misfolded forms of SOD1 (mSOD1) was tested.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
