Cat. No.
MABL-2970
Application
WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Eimeria acervulina
Clone No.
6D-12-G10
From
Recombinant Antibody
Specificity
This antibody recognizes and binds the apical complex of the sporozoite of Eimeria acervulina, which is a 20-21kDa protein.
Alternative Names
Sporozoite surface antigen; Eimeria acervulina
UniProt
Q24769
Immunogen
The original antibody was generated by immunizing chicken with sporozoite antigen of Eimeria acervulina, which is a 20-21 kDa protein.
Application Notes
Avain coccidiosis is caused by an intracellulae protozoan belonging to the genus Eimeria. This disease is important in aviculture due to its negative effects on production and development of birds. Avian coccidiosis is one of the main causes of growth retardation, poor feed conversion and high mortality in intensive farming of broilers, cage-free pullets and breeders. This mAb recognizes the apical complex of the sporozoite in a western blot assay and ELISA. Immunoelectron microscopic examination revealed that the antibody stained the conoid antigen. In indirect immunofluorescence assay the culture supernatent containing the mAb intensely stained the tip of the E. acervulina sporozoites. It is also reported that this antibody inhibited the invasion of sporozoites in CD8+ T cells in vitro (PMID: 8627507). The scFv antibody was produced at a minimum of 7 mg l−1 and exhibited virtually identical antigen reactivity as the original mAb (Min et al., 2001). mAb 6D-12-G10 shows cross reactivities in immunofluorescence staining at the apical end of sporozoites of C. parvum and C. muris, and merozoites of C. parvum (PMID:15725532).This mAb is also cross reactive with other species like Apicomplexan parasites, including other Eimeria spp., Toxoplasma, Neospora, and Cryptosporidium spp (PMID: 27384177)
Antibody First Published
Sasai et al. Characterization of a chicken monoclonal antibody that recognizes the apical complex of eimeria acervulina sporozoites and partially inhibits sporozoite invasion of cd8+ t lymphocytes in vitro. J Parasitol (1996); 82(1):82-7. PMID:8627507
Note on publication
Describes the generation of this antibody and its use in identifying the Eimeria sporozoite antigen that binds to CD8+ lymphocytes.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
