Cat. No.
MABL-2971
Application
crystallization, ELISA
Isotype
Engineer antibody
Species Reactivity
P. aeruginosa
Clone No.
ScFv5
From
Recombinant Antibody
Specificity
The antibody binds specifically to SpuE. In particular, it interacts with the hinge region that connects the two domains of SpuE.
Alternative Names
Spermidine-binding periplasmic protein SpuE
UniProt
Q9I6J0
Immunogen
The original antibody was isolated from a human scFv phage-displayed library by panning against SpuE.
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody conjugated to a membrane penetrating peptide (MPP) could inhibit the secretion of ExoS from P. aeruginosa. The antibody conjugated to MPP was able to inhibit the cytotoxic effects of SpuE in the human epithelial cell line A549. The antibody conjugated to MPP showed a protective effect on C. elegans from P. aeruginosa infection. The crystal structure of the antibody in complex with SpuE-spermidine was determined. The dissociation constant (KD) of SpuE to spermidine and of SpuE to spermidine in the presence of the antibody was measured by isothermal titration calorimetry (Kd = 2.7 nM and 29.8 nM, respectively) (Zhang et al., 2019; PMID: 31682834).
Antibody First Published
Zhang et al., A Potent Anti-SpuE Antibody Allosterically Inhibits Type III Secretion System and Attenuates Virulence of Pseudomonas Aeruginosa. J Mol Biol. 2019 Dec 6;431(24):4882-4896. doi: 10.1016/j.jmb.2019.10.026. Epub 2019 Nov 2. PMID:31682834
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
