Cat. No.
MABL-2974
Application
crystallize, RIA, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
A52
From
Recombinant Antibody
Specificity
A52 binds to both single- and double-stranded DNA (preference for the single-stranded conformation) as well as supercoiled DNA - has a preference for poly(dG) sequences. Poly(dA), poly(dC), poly(rG) and 23S rRNA do not bind to A52. Micelles of cardiolipin (structural analog of the sugar-phosphate backbone of nucleic acids) was shown to be non-inhibitory by ELISA. Both the sugar-phosphate backbone and the heterocyclic bases of DNA are required for antibody recognition. Anti-nucleic acid autoantibodies are found frequently in SLE patients as well as in mice strains such as NZB/NZW.
Alternative Names
single-stranded DNA; double-stranded DNA; supercoiled DNA
UniProt
Immunogen
A52 mAb was isolated by producing a hybridoma cell line using spleen cells derived from female unimmunized inbred, autoimmune NZB/NZW F1 mice, which serve as a model for human SLE (systemic lupus erythematosus).
Application Notes
Binding and specificity studies have been conducted with A52 by RIA and ELISA, using phage φχ 174 DNA, which provides ssDNA and dsDNA of identical sequence, and competition assays were performed using synthetic polynucleotides. The A52 Fab fragment used for sttructural studies was generated by digestion with papain. (PMID: 24449198)
Antibody First Published
Eilat et al. Monoclonal antibodies to DNA and RNA from NZB/NZW F1 mice: antigenic specificities and NH2 terminal amino acid sequences. J Immunol. 1984 Jul;133(1):489-94. PMID:6202791
Note on publication
Describes the characterization of the anti-DNA antibodies A52 and D42 - the binding kinetics and specificities were studied using the complete antibody and the individual reconstituted antibody chains. The initial sequence data was also determined.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
