Cat. No.
MABL-2975
Application
hyrdolysis, SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
m3D8
From
Recombinant Antibody
Specificity
Binds unspecifically to double- and single-stranded DNA oligomers.
Alternative Names
single-/double-strand DNA; desoxyribonucleic acid; dsDNA; ssDNA
UniProt
Immunogen
3`-biotin oligodeoxynucleotide ss(dN)40 with N=CCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAAC
Application Notes
This catalytic antibody binds to DNA oligomers both single- and double-stranded, irrespective of the sequence, showing DNA-hydrolytic activity. DNA is the principle storage of genetic information and usually sequestered within the nucleus in Eukaryotes. Extracellular DNA is a key danger signal for the immune system and an antigen to disease-causing auto-antibodies (incl. DNA-hydrolysing antibodies) e.g. in Systemic Lupus Erythematosus. Antibody formats comprising different numbers of variable domains may be useful tools in investigating the significance of these catalytic antibodies in disease.
Antibody First Published
Kim et al. 2006 Heavy and light chain variable single domains of an anti- DNA binding antibody hydrolyze both double- and single-stranded DNAs without sequence specificity. Journal of Biological Chemistry 2006; 281(22):15287-1595 PMID:16551636
Note on publication
Describes binding studies on mAB 3D8 on different types of oligos (ssDNA, dsDNA, dT:dA, dN:dN etc) using ELISA and SPR.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
