Cat. No.
MABL-2990
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
4D11
From
Recombinant Antibody
Specificity
This antibody shows high sensitivity for detection of sulfadoxine (SDM), sulfamonomethoxine (SMM), sulfamethoxazole (SMX), sulfamethazine (SMZ), and sulfaquinoxaline (SQX). It had high affinity for sulfisomidine (SIM), SMZ (including acetyl-SMZ), and sulfanitran (SNT). This antibody has higher affinity for the sulfonamides containing a six-atom-ring substitution (except sulfabenzamide -SBA and SDM) rather than a five atom-ring substitution at the N1 position for e.g. sulfamethizole (SMT) and sulfathiazole (STZ).Sulfonamides are synthetic bacteriostatic antibiotics that competitively inhibit conversion of p-aminobenzoic acid to dihydropteroate, which bacteria need for folate synthesis and ultimately purine and DNA synthesis.
Alternative Names
SDM; SMM; SMX; SMZ; SQX; sulfadoxine; sulfamonomethoxine ; sulfamethoxazole; sulfamethazine; sulfaquinoxaline
UniProt
Immunogen
The original antibody was generated by immunizing mice with a hapten made by covalently conjugating 4-(4-(4-aminophenylsulfonamido)phenyl)butanoic acid (SA10) with BSA.
Application Notes
The binding reactivity of this antibody for various sulphonamide drugs was determined using ELISA (PMID: 23417550). The binding affinity of this antibody towards various sulphonamide drugs is listed in table 1 of PMID: 30580515. This antibody was used in the development of broad-specific and sensitive competitive indirect ELISA for screening for sulfonamides. It used MAb 4D11 and the BS-BSA as heterologous-coating antigen and demonstrated a 50 % specific binding (IC50) for 22 sulfonamides at concentrations below 100 ng mL−1. The optimized ciELISA was used to quantify the five sulfonamides, SMZ, SDM, SQX, SMM, and SMX in spiked milk samples (Lian et al., Food Anal. Methods (2014)).
Antibody First Published
Wang et al. Monoclonal antibodies with group specificity toward sulfonamides- selection of hapten and antibody selectivity. Anal Bioanal Chem. 2013 May;405(12):4027-37. PMID:23417550
Note on publication
Describes the generation of antibodies with wide specificity for drugs with sulfonamide group.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
