Cat. No.
MABL-2989
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
4C7
From
Recombinant Antibody
Specificity
This antibody shows high sensitivity for detection of sulfamethizole (SMT) and sulfathiazole (STZ). It also has good sensitivity for detection of sulfachloropyridazine (SCP), sulfamethoxypyridazine (SMP), sulfamethoxazole (SMX), sulfaquinoxaline (SQX), sulfadoxine (SDM), sulfadimethoxypyrimidine (SDT) and sulfamoxole (SXL). Sulfonamides are synthetic bacteriostatic antibiotics that competitively inhibit conversion of p-aminobenzoic acid todihydropteroate, which bacteria need for folate synthesis and ultimately purine and DNA synthesis.
Alternative Names
STZ; SMT; Sulfathiazole; Sulfamethizole; ChEBI: 9337; ChEBI:9331
UniProt
Immunogen
The original antibody was generated by immunizing mice with a hapten made by covalently conjugating 2-(2-(4-aminophenylsulfonamido)thiazol-5-yl)acetic acid (TS) with BSA.
Application Notes
The binding reactivity of this antibody for various sulphonamide drugs was determined using ELISA (PMID: 23417550). The binding affinity of this antibody towards various sulphonamide drugs is listed in table 1 of PMID:30580515
Antibody First Published
Wang et al. Monoclonal antibodies with group specificity toward sulfonamides- selection of hapten and antibody selectivity. Anal Bioanal Chem. 2013 May; 405(12):4027-37. PMID:23417550
Note on publication
Describes the generation of antibodies with wide specificity for drugs with sulfonamide group.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
