Cat. No.
MABL-2988
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
8G1
From
Recombinant Antibody
Specificity
The antibody binds an epitope on sulfatase-2 polypeptide.
Alternative Names
Extracellular sulfatase Sulf-2; hSulf-2; Surf-2
UniProt
Q8IWU5
Immunogen
The original antibody was generated by immunizing sulf2 null mice with recombinant human SULF2.
Application Notes
This antibody was used for detection of Sulphatase-2 (SULF2), an extracellular sulfatase that acts on heparan sulfate proteoglycans and modulates multiple signaling pathways. The specificity of the monoclonal antibody was evaluated by ELISA, while sandwich ELISA was employed to detect the presence of SULF2 in blood and quantified its level.(PMID: 25444749) 8G1 reacted with the 75 kDa subunit of Sulf-2 and its 125 kDa proprotein precursor but not Sulf-1 by western blotting. s. The antibody was tested for its ability to revert the malignant phenotype of lung cancer cells (H292 cell line) in culture. The antibody markedly slow the growth of these cells on plastic dishes. 8G1 was tested for its effects on the growth of human lung cancer cells as xenograft tumors in immunocompromised host mice (nude mice), the antibody produced a marked inhibition of tumor size with time and in the final tumor weight (WO2012106192).
Antibody First Published
Singer et al. SULF2, a heparan sulfate endosulfatase, is present in the blood of healthy individuals and increases in cirrhosis. Clin Chim Acta. 2015 Feb 2; 0: 72–78. PMID:25444749
Note on publication
The paper describes the use of Sulphatase-2 (SULF2) as a serologic biomarker for liver cirrhosis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
