Cat. No.
MABL-2987
Application
ISB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
ID2E1
From
Recombinant Antibody
Specificity
This antibody is specific for keratin with an increased affinity for sulfur mustard-exposed (SM- exposed) keratin over unexposed keratin.
Alternative Names
UniProt
Immunogen
The original antibody was selected from existing phage display libraries.
Application Notes
The original format of this antibody (human scFv) demonstrated distinct recognition patterns in an ELISA and immunoslotblot (ISB) assay. Recognition of keratin exposed to increased concentrations of SM exhibited a maximal signal at 250 μM SM, indicating specific recognition of alkylated amino acid sequences. However, recognition was reduced at higher exposure levels (500 μM and 1 mM) but remained higher than that of unexposed keratin (Bikker et al., 2007; PMID: 17539823).
Antibody First Published
Bikker et al. Detection of sulfur mustard adducts in human callus by phage antibodies Chem Biol Drug Des. 2007 May;69(5):314-20. doi: 10.1111/j.1747-0285.2007.00504.x PMID:17539823
Note on publication
The original publication explores the application of phage display in the detection of sulfur mustard adducts in human callus, demonstrating the successful use of antibody phage display for immunodiagnostics of a chemical warfare agent. Furthermore, the publication describes the generation and characterization of clone ID2E1.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
