Cat. No.
MABL-3005
Application
ICC, IP, WB, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Chicken, Rat, Xenopus, Human, Mouse
Clone No.
8D4
From
Recombinant Antibody
Specificity
The antibody is specific for mammalian and avian talin. Talin is a multifunctional constituent of cell-substratum attachment sites and it has a high molecular weight (225-235 kDa). The protein is found in a variety of tissues and cell types.
Alternative Names
talin 1; Talin1; talin-1; TLN; TLN1
UniProt
Q9Y490
Immunogen
The original antibody was generated by immunizing BALB/c mice with purified cytoskeletal Talin, from chicken gizzard.
Application Notes
The antibody detected the N-terminal region of talin1 by western blot analysis (Praekelt et al., 2012; PMID: 22306379 and Martel et al., 2001; PMID: 11279249). However, it also detected the equivalent region of talin2, albeit to a lesser degree (Praekelt et al., 2012; PMID: 22306379). This antibody was used for detection of talin expression on HeLa/AT22, AT21, wild-type HeLa cells or pMEP4- transfected HeLa cells cells by flow cytometry (Albigès-Rizo et al., 1995; PMID: 7593292). lmmunocytochemistry using the antibody showed patterns of talin expression within DRG growth cones (Sydor et al., 1996; PMID: 8794861). Indirect immunofluorescence staining of cultured fibroblasts (rat, human, chicken and xenopus embryo fibroblasts) was tested with the antibody. In all the samples the focal adhesions were stained with the antibody (Otey et al. 1990; PMID: 1690175). Talin was immunoprecipitated from MDA-MB-231 cell lysates using the antibody (Beaty et al., 2014; PMID: 24891603).
Antibody First Published
Otey et al. Characterization of monoclonal antibodies to chicken gizzard talin Hybridoma. 1990 Feb;9(1):57-62. PMID:1690175
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
