Cat. No.
MABL-3006
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
T. aquaticus
Clone No.
TP7
From
Recombinant Antibody
Specificity
This antibody binds to Taq-Polymerase with a Kd of 1 nM.
Alternative Names
Taq DNA Pol; Thermostable DNA-Polymerase I
UniProt
P19821
Immunogen
Taq-Polymerase.
Application Notes
This antibody binds to Taq-polymerase, a thermostable DNA polymerase from a therophilic bacterium frequently used in PCR as its optimum temperature is about 75-80°C and is still active at temperatures up to 92.5°C. The antibody competes with DNA for Taq-binding.
Antibody First Published
Murali et al. Structural studies on an inhibitory antibody against Thermus aquaticus DNA polymerase suggest mode of inhibition. Protein Engineering 1998; 11(2):79-86 PMID:9605541
Note on publication
Describes the crystallisation of Taq-Polymerase with TP7 to identify the mechanism of inhibition and surface contacts.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
