Cat. No.
MABL-3016
Application
crystallisation, IP, FC, IF
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
Desire-1
From
Recombinant Antibody
Specificity
mAb desire-1 recognises an epitope contributed by both the alpha and beta chains of the KB5- C20 TCR.
Alternative Names
T cell receptor; T-cell receptor; Ti
UniProt
P01852; P01849
Immunogen
mAb desire-1 was obtained after fusion of spleen cells from a BALB.K mouse immunised twice with KB5-C20 clone cells in RPMI 1640 medium, with non-Ig-producing myeloma cells X63-Ag8.653. Desire-1 was selected for its capacity to inhibit selectively the H-2Kb-specific cytolysis by clone KB5-C20.
Application Notes
Desire-1 has been used for the immunoprecipitation of soluble TCR molecules (Gregoire 1991 & Hua 1986). An FITC conjugated desire-1 has been used in flow cytometric analysis as a marker for clonotype expression (Schonrich 1991). Further, a Fab fragment derived from desire-1 has been used in the crystallisation of the T-cell receptor for 3D structure determination (Housset 1997).
Antibody First Published
Hua et al. Lysis of hybridoma cells bearing anti-clonotypic surface immunoglobulin by clonotype-expressing alloreactive cytotoxic T cells Eur. J. Immunol. 1985. 15: 1029-1032 PMID:3902483
Note on publication
Describes the ability desire-1 to modulate the cytolytic activity of cytotoxic T lymphocyte clone KB5-C20.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
