Cat. No.
MABL-3017
Application
crystallisation, IP, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
H57-597
From
Recombinant Antibody
Specificity
mAb H57-597 reacts with surface receptors on all alpha-beta TCR bearing cells, but does not react with receptors on gamma-delta T cells. H57-597 is directed to an epitope within the FG loop of the C region of TCR beta chain.
Alternative Names
TCR-beta; T cell receptor; T-cell receptor; T cell receptor beta chain; TCRB; TRB; TCB1; Ti
UniProt
P01852
Immunogen
Hamsters were immunised with affinity purified DO-11.10 TCR, and produced detectable anti- TCR antibodies which were judged by the ability of the anti-sera to immunoprecipitate TCR from surface labelled DO-11.10 T cell hybridomas. Hamster 57 was selected for hybridisation, and B cell hybridomas were produced using polyethylene glycol fusion of hamster splenic B cells to the mouse myeloma variant. Selected mAb were screened after subsequent culturing for their ability to immunoprecipitate TCR from detergent lysates of surface-radiolabelled murine T cell hybridomas.
Application Notes
In an immobilised form, this antibody can directly activate T cells bearing alpha-beta TCR to produce IL-2. H57-597 has also been used to construct a Fab fragment which has been complexed with deglycosylated TCRs for their crystallisation for structure determination (Liu 1996). Flow cytometry is another application of this antibody, where H57-597 has been used to analyse TCR-beta expression in different T cell populations (Tsukumo 2006).
Antibody First Published
Kubo et al. Characterisation of a monoclonal antibody which detects all murine alpha-beta T cell receptors J Immunol. 1989 Apr 15;142(8):2736-42. PMID:2467936
Note on publication
Describes the need for a monoclonal antibody that detects alpha-beta but not delta- gamma TCRs, to allow enumeration of the two types of cells separately during development. The generation of the antibody is then outlined.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
