Cat. No.
MABL-3018
Application
ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
IARC307
From
Recombinant Antibody
Specificity
This antibody is specific for Vbeta8.1 CDR1 sequence CKPISGHNSLFQWYRQT.
Alternative Names
IARC-307; TCR-beta; T cell receptor; T-cell receptor; T cell receptor beta chain; TCRB; TRB; TCB1; Ti; TCR V beta; IARC/307; IARC 307
UniProt
P01850; A0A5B9
Immunogen
Derived from normal B cells in peripheral blood of a Burkitt’s lymphoma patient. Screened more than 60 culture supernatants from lymphoblastoid cell lines of normal origin, Epstein-Barr virus (EBV)- carrying Burkitt lymphomas, EBV-negative Burkitt lymphomas and EBV-converted sublines of original EBV- negative Burkitt lymphoma human B-cell lines for their capacity to bind the synthetic peptide autoantigens. One lymphoblastoid B-cell line was identified, IARC307, that produces IgM kappa antibodies which specifically react with the CDR1 peptide corresponding to human Vbeta8.1 gene sequence.
Application Notes
IARC307 has been used in ELISAs to bind to synthetic peptides of CRD1 region from several TCR Vbeta families of man and mouse (Marchalonis 2000).
Antibody First Published
Dedeoglu et al. Light and heavy chains specifying a human IgM kappa autoantibody to a T-cell receptor Vbeta-antigen Immunology Letters, 38 (1993)223-227 PMID:8125530
Note on publication
Describes the generation of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
