Cat. No.
MABL-3022
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Fab-8-1
From
Recombinant Antibody
Specificity
Binds to human TDRD3 with a Kd-value of 1 nM. Specificity checked by ELISA - it show no binding to any of 13 non-cognate antigens.
Alternative Names
Tudor-domain-containing protein 3
UniProt
Q9H7E2
Immunogen
Human TDRD3.
Application Notes
This antibody binds to the tudor domain of TDRD3, a nuclear coactivator that recognizes and binds asymmetric dimethylation on the core histone tails associated with transcirptional activation such as H4R3me2 or H3R17me2. In the cytoplasm, it is suggested to be involved in the assembly and disassembly of mRNA stress granules. Interesting feature about this antibody: The CDR-H3 plays virtually no role in antigen recognition.
Antibody First Published
Persson et al. Diversity is not required for antigen recognition by synthetic antibodies. Journal of Molecular Biology 2013; 425(4):803-811 PMID:23219464
Note on publication
Describes the generation of several synthetic antibodies from phage display and subsequent crystallisation with TDRD3.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
