Cat. No.
MABL-3028
Application
functional assay, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
3C4F5
From
Recombinant Antibody
Specificity
The antibody binds testosterone and shows reasonably low cross-reaction (~11.6%) with 5a- dihydrotestosterone, androstenedione and ~1% cross reaction with dehydroepiandrosteronesulphate (DHEAS). Testosterone is the primary sex hormone and anabolic steroid in males.
Alternative Names
ChEBI:17347
UniProt
Immunogen
The original mouse version of this antibody was raised by immunizing mice with a testosterone-3-carboxymethyloxime-thyroglobulin conjugate.
Application Notes
The original mouse IgG2b version of this antibody binds testosterone with an affinity of 0.3 nM. The binding properties of the antibody was preliminarily characterized by a competitive enzyme- linked immunosorbent assay. The combined (44 + 28) mutant derived from this antibody showed less cross- reactivity towards DHEAS of 0.033%, 44-fold less than in the wild-type (Hemminki et al, 1998; pmid:9680193). The combined mutant is available on request. To elucidate the structure of the mouse version of this antibody bound to testosterone x-ray crystallography was performed (Valjakka et al, 2002; pmid:11707437).
Antibody First Published
Hemminki et al. Specificity improvement of a recombinant anti-testosterone Fab fragment by CDRIII mutagenesis and phage display selection. Protein Eng. 1998 Apr;11(4):311-9. PMID:9680193
Note on publication
The specificity of a recombinant anti-testosterone Fab fragment was improved by CDRIII mutagenesis and phage display selection.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
