Cat. No.
MABL-3030
Application
functional assay, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
5F2
From
Recombinant Antibody
Specificity
The antibody is specific for the testosterone molecule. Testosterone is the primary sex hormone and anabolic steroid in males.
Alternative Names
ChEBI:17347
UniProt
Immunogen
This antibody was generated by panning against the VTT naïve human scFv phage display libraries VHVκ and VHVλ.
Application Notes
The testosterone binding affinity of the 5F2 Fab fragment is 900 nM as determined by a competitive ELISA. Binding specificity of the 5F2 Fab was also studied by using 5α‐ dihydrotestosterone, dehydroepiandrosterone sulphate and androstenedione. Furthermore the Fab version of this antibody was co-crystallized with testosterone and the structure was identified using x-ray crystallography (Niemi et al, 2011; pmid:21360611).
Antibody First Published
Niemi et al. The testosterone binding mechanism of an antibody derived from a naïve human scFv library. J Mol Recognit. Mar-Apr 2011;24(2):209-19. PMID:21360611
Note on publication
A testosterone binding scFv antibody was isolated from a naïve human library with a modest size of 100,000,000 clones.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
