Cat. No.
MABL-3031
Application
crystallography, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
77 Fab
From
Recombinant Antibody
Specificity
This antibody is specific for testosterone, a steroid hormone secreted by the testicles that acts as the principle male sex hormone and anabolic steroid.
Alternative Names
Fab77; Fab 77
UniProt
Immunogen
Testosterone.
Application Notes
This antibody binds to The original version of this antibody (mouse Fab) was shown to bind testosterone with a Kd of 0.3 nM and an ED50 of 4.8 nM (Valjakka et al., 2002; PMID: 12196551). The binding mechanism of this antibody was explored via crystallography (Niemi et al., 2011; 21360611).
Antibody First Published
Valjakka et al. Crystal structure of an in vitro-affinity- and specificity- matured anti-testosterone Fab in complex with testosterone. Improved affinity results from small structural changes within the variable domains. Journal of Biological Chemistry 2002; 277(46):44021-44027 PMID:12196551
Note on publication
The original publication describes the generation and structural analysis of a highly selective, high-affinity anti-testosterone Fab fragment, highlighting small structural changes within the variable domains that result in improved affinity and specificity through comprehensive packing of testosterone with the protein.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
