Cat. No.
MABL-3034
Application
functional assay, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
Nb16
From
Recombinant Antibody
Specificity
The antibody is specific for the testosterone molecule. Testosterone is the primary sex hormone and anabolic steroid in males.
Alternative Names
ChEBI:17347
UniProt
Immunogen
The antibody was raised by immunizing a young male bactrian camel with testosterone-BSA.
Application Notes
The binding characterization of this single domain VHH antibody was done using an ELISA on testosterone-BSA. Further a thermofluor experiment was used to determine the Tm value of the nanobody version of this antibody. A surface plasmon resonance was performed to test the binding affinity of the nanobody version of this antibody to testosterone (Li et al, 2016; pmid:27196036).
Antibody First Published
Guanghui Li et al. Generation of small single domain nanobody binders for sensitive detection of testosterone by electrochemical impedance spectroscopy. ACS Appl Mater Interfaces. 2016 Jun 8;8(22):13830-9. PMID:27196036
Note on publication
A phage display library of variable domain of the heavy chain only antibody or nanobody (Nb) was constructed after immunizing a bactrian camel with testosterone.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
