Cat. No.
MABL-3035
Application
functional assay
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
S83
From
Recombinant Antibody
Specificity
The antibody is specific for the testosterone molecule. Testosterone is the primary sex hormone and anabolic steroid in males.
Alternative Names
ChEBI:17347
UniProt
Immunogen
This antibody is an affinity matured version of the murine parent antibody 3C4F5. This antibody was generated by panning against a antibody phage library created using PCR mutagenesis.
Application Notes
The binding characterization of the antibody was done using ELISA. To show the effectiveness of this antibody, a competitive immunoassay was performed on the serum of unknown patients using the human version of this antibody. It was compared to a commercial testosterone kit. The results were comparable (WO1999012974). The original Fab fragment of this antibody, having 5 optimised CDRs, showed 32-fold higher relative affinity to testosterone, 60-fold lower cross-reactivity to DHEAS, 2 fold lower cross-reactivity to 5α-DHT and over 9-fold lower cross-reactivity to androstenedione compared to the original monoclonal antibody (Ari Hemminki, 1998; ISBN 951-38-5343-8).
Antibody First Published
Ari Hemminki Development of recombinant antibodies for diagnostic applications by protein engineering. ISBN 951-38-5343-8
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
