Cat. No.
MABL-3036
Application
functional assay, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
TM-4
From
Recombinant Antibody
Specificity
The antibody is specific for the testosterone molecule. Testosterone is the primary sex hormone and anabolic steroid in males.
Alternative Names
ChEBI:17347
UniProt
Immunogen
This antibody was raised by panning against a library that has earlier been selected twice for activity against testosterone-biotin(testosterone3-(5-(6-(5- (biotin)pentanamido)hexanamido)pentylcarbamoyl) propanoate) coupled to avidin-coated magnetic beads.
Application Notes
The binding characteristics of the original version of this antibody (human scFv) to testosterone and biotinylated testosterone were characterized via competitive ELISA. It exhibited an IC50 value of 2 and 4 nM for testosterone and biotinylated testosterone, respectively (Persson et al., 2008; PMID: 18480091).
Antibody First Published
Persson et al. In vitro evolution of an antibody fragment population to find high-affinity hapten binders. Protein Eng Des Sel. 2008 Aug;21(8):485-93. PMID:18480091
Note on publication
The original publication details the in vitro evolution of an antibody fragment population, focusing on enhancing their hapten binding properties, particularly targeting testosterone as a model antigen, resulting in a considerable increase in affinity and the identification of novel variants with different fine-specificities.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
