Cat. No.
MABL-3045
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
T3-15
From
Recombinant Antibody
Specificity
The antibody is highly selective for Tetrabromobisphenol A and it shows negligible cross reactivity (<0.1%) with other tested analogues (TBBPA-bAE, TBBPA-bOHEE, DBDPE, HBCD, BDE-47, BDE-99, BDE-100, BDE-153, BDE-154, BDE-183, 5-OH-BDE 47, 6-OH-BDE 47, BPA). Tetrabromobisphenol A is a ubiquitous brominated flame retardant.
Alternative Names
TBBPA; 79-94-7; 3,3',5,5'-Tetrabromobisphenol A; Bromdian; 4,4'-(propane-2,2-diyl)bis(2,6-dibromophenol); 2,6-dibromo-4-[2-(3,5-dibromo-4-hydroxyphenyl)propan-2-yl]phenol; 2,2-bis(3,5-dibromo-4-hydroxyphenyl)propane; T15; brominated flame retardant
UniProt
Immunogen
The original antibody was generated by immunizing an alpace with a TBBPA hapten coupled to thyroglobulin. A VHH phage display library was constructed with the blood lymphocytes and panning against heterogeneous coating antigens.
Application Notes
The VHH fragment was used to develop an ELISA for monitoring environmental and human exposure to TBBPA. This assay had an IC50 for TBBPA of 0.40 ng/mL. Thermal stability studies showed the VHH−antigen binding signal retained about 80% and 20% of the unheated control signal after heating at 90 °C for 10 and 90 min, respectively. The recoveries of TBBPA from spiked soil and fetal bovine serum samples showed good correlation with liquid chromatography−tandem mass spectrometry method (Wang et al, 2014; PMID: 25068372). The VHH fragment was fused with alkaline phosphatase (AP) and a one-step immunoassay for the detection of TBBPA was developed, showing a half-maximum signal inhibition concentration (IC50) of 0.20 ng/mL. Compared to the parental VHH, the VHH-AP was able to bind to a wider variety of coating antigens and the assay sensitivity was slightly improved (Wang et al., 2015; PMID: 25849972).
Antibody First Published
Wang et al. Heterologous Antigen Selection of Camelid Heavy Chain Single Domain Antibodies against Tetrabromobisphenol A Anal. Chem. 2014, 86, 16, 8296–8302 PMID:25068372
Note on publication
The paper describes the generation and characterization of the antibody. The antibody was used to develop an enzyme-linked immunosorbent assay for monitoring environmental and human exposure to TBBPA.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
