Cat. No.
MABL-3046
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
scFvTc
From
Recombinant Antibody
Specificity
This antibody binds tetracycline. Tetracycline, is an antibiotic derived from microorganisms of the genus Streptomyces and are broadly used to treat bacterial infections. This antibody is reported to cross-react with aureomycin and also shows weak reactivity for oxytetracycline and doxycycline.
Alternative Names
Tc; antibiotic; tetracycline hydrochloride; aureomycin; oxytetracycline; doxycycline
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with tetracycline hydrochloride-BSA as an immunogen. The cDNA obtained from total mRNA of the immunized mouse splenocytes was used as a template to amplify the variable domain genes and generate an scFv phage display library. Later on the antibody was selected by panning this library against tetracycline-ovalbumin conjugate.
Application Notes
The binding characterization of this antibody for tetracycline was done using ELISA. Additionally, this antibody was also capable of cross-reacting with aureomycin, oxytetracycline and doxycycline in an ELISA. The colloidal gold labelled version of this antibody was used for the development of colloidal gold test strip for detection of tetracycline hydrochloride (CN103396484).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
