Cat. No.
MABL-3047
Application
ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
4/12
From
Recombinant Antibody
Specificity
This antibody is specific for the largest extracellular domain of tetraspanin 1.
Alternative Names
Net1; C4.8; Tetraspanin-1; Tspan-1; TSPAN1; Tetraspan NET-1; Tetraspanin TM4-C; TM4SF
UniProt
O60635
Immunogen
This antibody was raised by immunising BALB/c mice with the major extracellular domain of tetraspanin 1.
Application Notes
The specificity of this antibody for tetraspanin 1 has been confirmed in ELISA analysis (Hölters et al, 2013). This antibody has been used in immunohistochemical analysis of tetraspanin 1 expression in paraffin-embedded sections of normal and cancerous cervical tissue arrays (Hölters et al, 2013), and in frozen tissue samples. This antibody has also been used for immunocytochemical staining for tetraspanin 1 expression, in SiHa cells following adenoviral transduction (Hölters et al, 2013).
Antibody First Published
Hölters et al. Tetraspanin 1 promotes invasiveness of cervical cancer cells Int J Oncol. 2013 Aug;43(2):503-12. PMID:23754316
Note on publication
Describes the original generation of this antibody, and its use in immunohistochemical analysis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
