Cat. No.
MABL-3057
Application
SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
C4C4
From
Recombinant Antibody
Specificity
This antibody binds thiacloprid, which is a neonicotinoid insecticide. It is used in the production of a lot of agricultural crops and acts by affecting the insects nervous system. The antibody is also reported to have some cross reactivity with imidaclothiz and acetamiprid, slight cross reactivity with 2- chloro-5-[[2-(nitromethy-l-ene)-1-imidazolidinyl] methyl] pyridine (6-Cl-PMNI) and the thiacloprid amide (around 2%), and no obvious cross reactivity (<2%) with other structural analogues.
Alternative Names
THD; neonicotinoid; pesticide; insecticide; CAS no: 111988-49-9; ChEBI: 391756; {(2Z)-3-[(6-Chloropyridin-3-yl)methyl]-1 3-thiazolidin-2-ylidene}cyanamide
UniProt
Immunogen
The original antibody was generated by immunizing 6 weeks old BALB/c mice with a thiacloprid–bull serum albumin (BSA) conjugate.
Application Notes
This antibody is recommended for detected of thiacloprid and other neonicotinoid pesticides. The binding characterization of this antibody to thiacloprid was done using ELISA. The recombinant antibody showed a binding profile similar to that of the original parental antibody. The reactivity of this antibody to other neonicotinoid pesticides was also determined using surface plasmon resonance. It was observed that thiacloprid and imidaclothiz displayed high binding responses, while imidacloprid, acetamiprid, and clothianidin showed medium binding responses. No significant binding was found toward dinotefuran, nitenpyram, and thiamethoxam, similar to the negative controls of two organophosphorus pesticides. (PMID: 32962080).
Antibody First Published
Liu et al. Characterization of Variable Region Genes and Discovery of Key Recognition Sites in the Complementarity Determining Regions of the Anti-Thiacloprid Monoclonal Antibody. Int J Mol Sci. 2020 Sep; 21(18): 6857. PMID:32962080
Note on publication
Describees the use of next generation sequencing to characterize the variable regions of the antibody and a method of generation of sequence defined recombinant antibodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
