Cat. No.
MABL-3058
Application
Complement‑dependent cytotoxicity, glycan array technologies, in vitro, in vivo, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
KT-IgM-8
From
Recombinant Antibody
Specificity
The antibody is specific for Tn-PS A1. It does not cross react with PS A1.
Alternative Names
Tn-PS A1; Thomsen-nouveau (Tn); Tn antigen; Tn saccharide; N-acetyl Galactosamine; Alpha-GalNAc; Alpha GalpNAc; GalNAc; GalNAc-alpha; N-acetyl-alpha-D-galactosamine; Blood group A antigen
UniProt
Immunogen
The original antibody was generated by immunizing C57BL/6 mice against Tn-PS A1.
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis. The ability of the antibody to bind cancer cells expressing the Tn antigen on the cell surface (MCF-7 and HCT-116) was evaluated by by FC. The antibody demonstrated the ability to bind both MCF-7 and HCT-116 tumor cells at 30 µg/mL with a shift in fluorescence of 49% in both cell lines. The antibody demonstrated in vitro tumor killing using an LDH assay. The assay measured that the antibody-induced complement- dependent cytotoxicity was 30%. In vivo passive immunotherapy approach using an MCF-7 cell line-derived xenograft model was evaluated. The antibody demonstrated approximately 40% reduction in tumor growth in the MCF-7 model (Trabbic et al., 2018; PMID: 30030557).
Antibody First Published
Trabbic et al. Production of a mouse monoclonal IgM antibody that targets the carbohydrate Thomsen-nouveau cancer antigen resulting in in vivo and in vitro tumor killing Cancer Immunol Immunother. 2018 Sep;67(9):1437-1447. PMID:30030557
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
