Cat. No.
MABL-3093
Application
WB, Block, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
5G9
From
Recombinant Antibody
Specificity
Alternative Names
TF; platelet tissue factor; factor III; thromboplastin; CD142
UniProt
P13726
Immunogen
Human TF.
Application Notes
The antibody binds specifically to TF, a hemostatic protein which is involved in the extrinsic pathway of coagulation. TF is receptor for circulating serine protease factor VIIa, activating the catalytic VIIa protease domain. The complex formed functions to activate the zymogen factor X into activated factor Xa, which then catalyses the formation of Prothrombin to Thrombin. The pathways results in a fibrin clot which acts to stop bleeding. The monoclonal antibody 5G9 acts as a competitive inhibitor of factor X, thereby inhibiting its activation by the TF.VIIa complex and the formation of a clot.
Antibody First Published
Ruf W, Edgington TS. An anti-tissue factor monoclonal antibody which inhibits TF.VIIa complex is a potent anticoagulant in plasma. Thromb Haemost. 1991 Nov 1;66(5):529-33. PMID:1803616
Note on publication
Describes the production of a murine monoclonal antibody directed against TF, and inhibits the complex of TF which Factor VIIa, thereby acting as an anticoagulant in plasma.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
