Cat. No.
MABL-3094
Application
functional assay, neutralize, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
ATR-5
From
Recombinant Antibody
Specificity
This antibody binds human tissue factor (TF) a hemostatic protein which is involved in the extrinsic pathway of coagulation. TF is receptor for circulating serine protease factor VIIa, activating the catalytic VIIa protease domain. The complex formed functions to activate the zymogen factor X into activated factor Xa, which then catalyzes the formation of Prothrombin to Thrombin. The pathways results in a fibrin clot which acts to stop bleeding. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Alternative Names
CD142; TF; hTF; F3; Coagulation factor III; Thromboplastin; platelet tissue factor; factor III
UniProt
P13726
Immunogen
The original antibody was generated by immunizing 5 weeks old male BALB/c mice subcutaneously into the abdomen with purified human tissue factor in equal quantity of Freund's complete adjuvant.
Application Notes
The binding of this antibody to human tissue factor expressed on the surface of human bladder carcinoma cells J82 was done using whole cell ELISA. This neutralizing activity of this antibody against the FX-binding was measured using the human placenta-derived thromboplastin. This antibody was also capable of inhibiting plasma coagulation (US6677436).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
