Cat. No.
MABL-3095
Application
functional assay, neutralize, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
D3H44
From
Recombinant Antibody
Specificity
This antibody binds human tissue factor (TF) a hemostatic protein which is involved in the extrinsic pathway of coagulation. TF is receptor for circulating serine protease factor VIIa, activating the catalytic VIIa protease domain. The complex formed functions to activate the zymogen factor X into activated factor Xa, which then catalyzes the formation of Prothrombin to Thrombin. The pathways results in a fibrin clot which acts to stop bleeding. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Alternative Names
CD142; TF; hTF; F3; Coagulation factor III; Thromboplastin; platelet tissue factor; factor III; D3
UniProt
P13726
Immunogen
The original antibody was generated by grafting CDRs of parental murine antibody D3 onto human framework regions. Using computer graphics model of the murine D3 heavy and light chain sequences several amino acid residues in the CDRs as well as framework region of light and heavy chains were altered using site-directed mutagenesis in order to optimize antigen binding.
Application Notes
This antibody is the humanized and affinity matured version of murine antibody D3. This antibody binds human tissue factor with a 100-fold increased affinity (KD 0.1 nM.) as compared to the original murine and chimeric versions. In vitro, D3H44 displays potent inhibition of plasma clotting, prevents FXa activation through the TF/FVIIa pathway (IC50 = 47 pM) and prevents fibrin deposition in a human ex-vivo thrombosis model (IC50 = 37 nM.) (PMID: 11307801). In vivo studies with this antibody demonstrated efficient antithrombotic activity. Anti-TF antibodies may prove useful in cardiovascular disorders and cancer, given the role of TF in these diseases (PMID: 15379713). This antibody was used in the immunofluorescence staining of tissue factor expressed on the surface of SW480 colon cancer cells. Fibrin formation was observed in plasma after 200 secs (PMID: 30462538).
Antibody First Published
Presta et al. Generation of a humanized, high affinity anti-tissue factor antibody for use as a novel antithrombotic therapeutic. Thromb Haemost. 2001 Mar;85(3):379-89.PMID:11307801
Note on publication
Describes the generation and characterization of humanized version of murine anti- tissue factor antibody D3.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
