Cat. No.
MABL-3102
Application
Blocking, IP, WB, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
TL2.1
From
Recombinant Antibody
Specificity
This antibody is specific to TLR2 (extracellular domain) and does not crossreact with human TLR1 or TLR4. TLR2 is involved in the immunological response against bacterial lipoproteins and other microbial cell wall components.
Alternative Names
CD282; Toll-like receptor 2; Toll/interleukin-1 receptor-like protein 4; human Toll like receptor 2
UniProt
O60603
Immunogen
This antibody was raised by immunising BALB/c mice with CHO cells transfected with human TLR2 cDNA.
Application Notes
TL2.1 antibody has been reported as an effective inhibitor of TLR2-dependent cytokine production and might be used to control and manipulate signalling pathways related to TLR2 (Flo et al., 2000). This antibody has also been exstensively used in flow cytometry and TL2.1 is recommended for the use in flow cytometric investigation of TLR2 expression (Jamin et al., 2015; Wang et al., 2016). For instance, one group treated dendritic cells with azithromycin and observed that this substance supressed TLR2 expression detected via flow cytometry (Lin et al., 2016). Finally, this antibody has been successfully applied in immunofluorescence analysis of TLR2 expression in various cells. One group utilised TL2.1 antibody to investigate TLR2 expression (among others pathogen-related receptors) on SRIK-NKL cells. CD8+ NK/T cell line (Srivastava and Srivastava, 2005).
Antibody First Published
Flo et al. Human toll-like receptor 2 mediates monocyte activation by Listeria monocytogenes, but not by group B streptococci or lipopolysaccharide. J Immunol. 2000 Feb 15;164(4):2064-9. PMID:10657659
Note on publication
This article describes the generation and characterisation of TL2.1 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
