Cat. No.
MABL-3111
Application
Agnostic, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
133-E12
From
Recombinant Antibody
Specificity
This antibody is specific for human TLR4.
Alternative Names
hToll; CD284; Toll-like receptor 4
UniProt
O00206
Immunogen
The original antibody was generated by immunizing llamas with a cocktail of murine and human Toll-like receptors (TLRs) that contained recombinant human TLR4/MD2, recombinant human TLR3, recombinant murine TLR3, and recombinant murine TLR2/Fc chimera. Peripheral blood mononuclear cells were isolated from the animals' blood for the construction of a phage display library, and the library was panned against the TLRs used for immunization.
Application Notes
The binding specificity of the original version of this antibody (VHH) was tested using ELISA. In a cell-based neutralizing assay with hTLR4-overexpressing HEK cells, an agonistic effect was observed, as measured by increased NFκB expression after cell lysis compared to the control group, indicating activation or enhancement of TLR activity instead of neutralization (WO2009138494A2).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
