Cat. No.
MABL-3140
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
CSF-scFv
From
Recombinant Antibody
Specificity
This antibody is specific for human TPI.
Alternative Names
TPI; TPI1; TIM; Methylglyoxal synthase; Triose-phosphate isomerase; EC:5.3.1.1; EC:4.2.3.3
UniProt
P60174
Immunogen
The original antibody was generated by assembling immunoglobulin VH and VL genes from clonal B cells found in the cerebrospinal fluid (CSF) of patients with clinically isolated syndrome (CIS).
Application Notes
This antibody's original format (human scFv) was used in an ELISA and WB assay (Kolln et al., 2006; PMID: 17015754).
Antibody First Published
Kolln et al. Triosephosphate isomerase- and glyceraldehyde-3-phosphate dehydrogenase-reactive autoantibodies in the cerebrospinal fluid of patients with multiple sclerosis. J Immunol. 2006 Oct 15;177(8):5652-8. doi: 10.4049/jimmunol.177.8.5652. PMID:17015754
Note on publication
The original publication investigates the presence of autoantibodies, including single-chain variable fragment antibodies (scFvs) generated from clonal B cells in the cerebrospinal fluid and lesions of multiple sclerosis (MS) patients, targeting the glycolytic enzymes triosephosphate isomerase (TPI) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
