Cat. No.
MABL-3141
Application
IF
Isotype
Engineer antibody
Species Reactivity
Clone No.
Jel 466
From
Recombinant Antibody
Specificity
Jel 466 binds to triple-stranded DNA. The preferred interacting partner is the triplex form of poly[d(TC)]. poly[d(GA)] and there is also good binding to poly[d(TTC)].poly[d(GAA)]. Theantibody binds weakly to the triplex derived from poly[d(G)].poly[d(C)] and no binding is observed with poly[d(T)].poly[d(A)1.poly[d(T)]. Jel 466 does not bind to poly[d(TC)].poly[d(Gm6A)], single-stranded DNAs, calf thymus DNA, or to poly[d(TG)].poly[d(CA)] which are unable to form a triplex structure. Triplex DNA has been known to form in vitro from synthetic pyrimidine-pruine tracts and it is also thought to potentially have a biological function in cells. These functions include the control of gene expression due to the presence of pyrimidine-purine tracts in the 5' control region and chromosomal organization whereby DNA loops can be stabilised by triplexes (some MARs [matrix attched regions] contain triplez-forming sequences).
Alternative Names
triple helix DNA; Jel-466; Jel466
UniProt
Immunogen
Jel 466 was prepared by immunizing mice with poly[d(Tm5C)].poly[d(GA)].
Application Notes
Jel 466 can be used to detect triplex DNA in cells by IF.
Antibody First Published
Agazie et al. Characterization of a new monoclonal antibody to triplex DNA and immunofluorescent staining of mammalian chromosomes. J Biol Chem. 1994 Mar 4;269(9):7019-23. PMID:7509814
Note on publication
Describes the use of Jel 466 to study the distribution of triplex DNA in vivo and compares Jel 466 to another anti-triplex DNA antibody, Jel 318.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
