Cat. No.
MABL-3142
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Pig
Clone No.
6C6D7
From
Recombinant Antibody
Specificity
This antibody binds tritrpticin (VRRFPWWWPFLRR), which is a member of the cathelicidin family, a 13mer tryptophan-rich group of diverse cationic antimicrobial peptides found in neutrophil granules. It has a broad spectrum activity against a range of microorganisms, fungi, and protozoa.
Alternative Names
C6; Prophenin and tritrpticin precursor
UniProt
P51524
Immunogen
The original antibody was generated by immunizing female BALB/c mice of 6-8 weeks of age with 200 µg of K-tritrpticin-diphtheria toxoid conjugate emulsified with equal volume of complete Freund’s adjuvant.
Application Notes
The binding characterization of this antibody was done using ELISA. In an indirect- binding ELISA where the peptides were directly immobilized on to the ELISA plate, mAb 6C6D7 could bind to tritrpticin and its N-terminal deleted antibacterial analogs at nanomolar concentrations. The binding affinity of this antibody to K-tritrpticin was analyzed using surface plasmon resonance and it was reported to be Kd= 350 nM. The binding of 6C6D7 to tritrpticin is characterized by negligible change in ΔGeq with respect to temperature that suggests that the antibody does not undergo any major conformational alteration during the binding of peptide antigen. This antibody was also used to bind tritripticin and related peptides to further analyze their anti-microbial activity (PMID: 24086578).
Antibody First Published
Sharma et al. Putative bioactive motif of tritrpticin revealed by an antibody with biological receptor-like properties. PLoS One. 2013 Sep 24;8(9):e75582. PMID:24086578
Note on publication
Describes the generation of this antibody to study the mechanism of action of this antimicrobial peptide.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
