Cat. No.
MABL-3143
Application
in vivo, therapeutic, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
hRS7 (Sacituzumab)
From
Recombinant Antibody
Specificity
This antibody binds human TROP2 (EGP-1) protein, which functions in a variety of cell signaling pathways and was first elucidated as a transducer of an intracellular calcium signal. Trop-2 is involved in several cell signaling pathways, of which many are associated with tumorigenesis. This antigen is found in many epithelial cancers, including carcinomas of the lung, bladder, breast, cervix, ovary, stomach, prostate cancer, and therefore this antibody could be suitable for targeting these cancers.
Alternative Names
EGP-1; TACSTD2; GA733-1; M1S1; TROP-2; Tumor-associated calcium signal transducer 2; epithelial glycoprotein-1 antigen; Cell surface glycoprotein Trop-2; Membrane component chromosome 1 surface marker 1; Pancreatic carcinoma marker protein GA733-1; IMMU-132; RS7; RS7-3G11
UniProt
P09758
Immunogen
The parental mouse antibody RS7-3G11 was generated by immunizing mice with crude membrane preparation of a human primary squamous cell carcinoma from the lung. The humanized version of this antibody called hRS7 was generated by grafting CDRs of the parental mouse antibody onto human framework regions.
Application Notes
The human IgG1 version of this antibody labelled with 131I-IMP-R4 was evaluated for the preclinical radioimmunotherapy (RAIT) of breast cancer. It was used for in vivo experiments in nude mice bearing subcutaneous MDA-MB-468 human breast cancer xenografts (PMID: 14999147). This antibody was used in the immunohistochemical staining of frozen sections of PC3 tumor xenografts. 111In-hRS7 and 89Zr-hRS7 labelled version of this antibody were used for the visualization of PC3 tumors in nude mice using immuno-PET and immuno-SPECT (PMID: 21865288). This antibody was also used in flow cytometric studies for detecting TROP-2 expressed on the surface of primary ovarian cancer cell lines. It was reported that primary ovarian carcinoma cell lines are highly sensitive to hRS7-mediated cytotoxicity in vitro (PMID:21453957).
Antibody First Published
Govindam et al. Preclinical therapy of breast cancer with a radioiodinated humanized anti-EGP-1 monoclonal antibody: advantage of a residualizing iodine radiolabel. Breast Cancer Res Treat. 2004 Mar;84(2):173-82. PMID:14999147
Note on publication
Describes the generation of humanized version of anti-EGP-1 antibody EGP-1 hRS7, labeled with 131I-IMP-R4 and evaluates its use in preclinical radioimmunotherapy (RAIT) of breast cancer.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
